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Photoacoustic technique is a pump-probe technique popularly used for many deep biomedical imaging applications. Due to the bulkiness of the setup and high cost, it would not be suitable for clinical research in India. We have recently developed a compact continuous wave laser diode based photoacoustic setup that can be easily field deployable with reduced cost. Here we propose to use a compact, cost effective, indigenously developed Continuous Wave Photoacoustic Spectral Response (CWPASR) technique to investigate the differences between tissues of different organs. The aim of the study is to utilize the mechano-biological properties of the tissue through the acoustic frequency (spectral) information. The zebrafish fixed tissue sample was used as an experimental model in this paper. The results obtained through CWPASR were compared with the conventional (pulsed laser based) PASR technique and were found to be identical. Hence the proposed technique has potential to become a regular tool for many clinical applications.
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