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In recent decade Photoacoustic (PA) has emerged as a valuable tool for optical and thermal characterization of a wide range of materials like biological, chemical and even geological materials, offering significant improvement over conventional spectroscopic technique. This rapidly growing multidisciplinary field of research brings together physicists; chemist; biologists; agricultural, food, environmental and medical scientist; and so on. PA spectroscopy is based on the principle of detection of non-radiative relaxation energy, resulting when atoms/molecules of the materials are excited from the ground state to higher energy states, by periodic absorption of electromagnetic radiations. Spectroscopy of delicate samples (such as biological materials.) is an area of immediate interest to medical scientist. In present talk I will present our observation on the study of bio-materials such as Gall bladder stones. Spectral characteristics of radiation absorbed by gall bladder stones can provide a more through understanding of the physical response to its initiation in the human body. We have recorded the PA spectra of gall bladder stones to know its molecular compositions. We have also recoded the LASER Induced Breakdown Spectra to know its elemental compositions. A detail observation on this study will be presented in this talk.
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